Isozymes of cyclic-3′,5′-nucleotide phosphodiesterases in renal epithelial LLC-PK1 cells
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چکیده
منابع مشابه
Gentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells
Nephrotoxicity is a major limiting factor in the use of aminoglycoside antibiotics, the mechanisms for which are still speculative. To clarify the mechanisms of renal tubular cell death induced by aminoglycosides, we examined the renal proximal tubule-like cell line, LLC-PK1, after inducing apoptosis through a chronic treatment with gentamicin (GM). Changes in the expression of the Fas were als...
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L-Histidine and imidazole (the histidine side chain) significantly increase cAMP accumulation in intact LLC-PK1 cells. This effect is completely inhibited by isobutylmethylxanthine (IBMX). Histidine and imidazole stimulate cAMP phosphodiesterase activity in soluble and membrane fractions of LLC-PK1 cells suggesting that the IBMX-sensitive effect of these agents to stimulate cAMP formation is no...
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Nephrotoxicity is one of the important adverse effects induced by cisplatin (CDDP), a potent anticancer drug. CDDP causes a disorder of renal proximal tubules. So, we evaluated mechanisms of renal tubular injury induced by CDDP using established epithelial cell line, LLC-PK1, which is characteristic of renal proximal tubular epithelium. LLC-PK1 cells were incubated in culture media except CDDP ...
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The presence and properties of cyclic 3',5'-adenosine monophosphate phosphodiesterase (cAMP-PDIE) and cyclic 3',5'-guanosine monophosphate phosphodiesterase (cGMP-PDIE) were studied in glomeruli isolated from rat renal cortex by sieving and density gradient centrifugation. The specific activity of cGMP-PDIE was higher than the specific activity of cAMP-PDIE in glomeruli; in tubules and renal co...
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Two distinct transporters for nucleobases have been characterized in LLC-PK1 cells. The first system accumulates hypoxanthine against a concentration gradient in the presence of sodium. The sodium-dependent uptake of hypoxanthine was saturable at 22 degrees C with a Km value of 0.79 +/- 0.43 microM, a Vmax of 15 +/- 4 pmol/mg protein/60 s, and a Na+:hypoxanthine coupling stoichiometry of 1.27 +...
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ژورنال
عنوان ژورنال: Kidney International
سال: 1992
ISSN: 0085-2538
DOI: 10.1038/ki.1992.12